Molecular Cloning and Characterisation of Phosphoglycerate Kinase from Fasciola Hepatica
نویسندگان
چکیده
This paper is focused on cloning and bioinformatical as well as immunological characterisation of the new vaccine antigen candidate against fasciolosis – Fasciola hepatica phosphoglycerate kinase (FhPGK). The antigen was cloned from the adult fluke by the use of RACE-PCR method. It was found that FhPGK is not a secretory and not a stage specific protein. It is present in all kinds of parasite tissue, particularly in fluke intestine and tegumental as well as subtegumental layers. FhPGK is involved in production of the first ATP molecule in the glycolytic pathway and can be used in vaccination trials in which the strategy is to block fluke`s energy metabolism. This is the first, to date, phosphoglycerate kinase cloned from F. hepatica.
منابع مشابه
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